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Molecular Ecology: Early molecular responses of coral larvae to hyperthermal stress by Rodriguez-Lanetty, Harii, Hoegh-Guldberg

01/05/2010 admin Leave a comment

http://www3.interscience.wiley.com/journal/122680126/abstract?CRETRY=1&SRETRY=0

Early Molecular responses of coral larvae to hyper thermal stress

Published in Molecular Ecology

Volume 18 Issue 24, Pages 5101 – 5114

Published Online: 9 Nov 2009

MAURICIO RODRIGUEZ-LANETTY*, SAKI HARII† and OVE HOEGH-GULDBERG‡

*Department of Biology, University of Louisiana at Lafayette, Lafayette, LA 70504, USA , †Graduate School of Engineering and Science, University of Ryukyus, 1 Senbaru, Nishihara Okinawa, 903-0213, Japan , ‡Centre for Marine Studies, University of Queensland, St. Lucia, Qld 4072, Australia

Correspondence to M. Rodriguez-Lanetty, Fax: 337 482 5834; E-mail: rodriguez-lanetty@louisiana.edu

KEYWORDS

climate change • coral larvae • coral stress response • ecological genomics • microarray

ABSTRACT

Most of the work on the impact of elevated temperature and light on Symbiodinium-invertebrate symbioses have focused primarily on how the photosynthetic (algal) partner is impacted. Understanding how the same stresses affect the invertebrate host, however, is in its infancy. In this study, we re-examined the direct effect of elevated temperatures on the invertebrate host exploring the early transcriptional response of aposymbiotic (without algal symbionts) coral larvae. The temperatures tested in the experimental design were 24 °C (ambient seawater temperature), 28 °C and 31 °C; and the sampling points were 3 and 10 h after temperature exposure. We explored relative changes in transcription using a cDNA microarray constructed for the scleractinian coral, Acropora millepora, and containing 18 142 expressed sequence tag (EST) clones/8386 unigenes. Our study identified 29 genes that were significantly up- and down-regulated when A. millepora coral larvae were exposed to elevated temperatures. Down-regulation of several key components of DNA/RNA metabolism was detected implying inhibition of general cellular processes. The down-regulation of protein synthesis, however, was not simple and random, which suggested that the stress response was a more complicated adjustment of cellular metabolism. We identified four significant outcomes during the very early hours of the transcriptional response to hyperthermal stress in coral larvae. First, the expression of heat-shock proteins increased rapidly (within 3 h) in response to hyperthermal stress. Second, a fluorescent protein homologue, DsRed-type FP, decreased its expression in response to elevated temperature reinforcing a potential role as a molecular marker for monitoring hyperthermal stress in nature. Third, the down-regulation of a coral mannose-binding C-type lectin under elevated temperature suggests that heat stress might compromise some components of the coral immune defence and therefore might bring about susceptibility to pathogenic diseases. And last, genes involved in protecting cells against oxidative stress showed little response at the early hours to heat stress, supporting the proposal that up-regulation of cnidarian host oxidative stress genes may require reactive oxygen species generated by stressed algal symbionts.

Received 11 March 2009; revision received 30 September 2009; accepted 2 October 2009

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Bulletin of Marine Science: Eutrophication and Trophic State Classification of Seagrasses in the Florida Keys by B.E. Lapointe, et. al.

01/01/2010 admin Leave a comment

Eutrophication

Brian E. Lapointe, David A. Tomasko, and William R. Matzie
Bulletin of Marine Science, 54(3) 696-717, 1994
ABSTRACT

“Cultural eutrophication is the most frequently cited factor correlating with the marked global decline in areal extent and vigor of seagrass communities over the past two decades.” — (Larkum, 1976; Kemp et al.;1983; Cambridge and McComb, 1984; Orth and Moore, 1984; Bourcier, 1986; Siberstein et al., 1986; Valiela et al., 1990; Green et al., 1990, Tomasko and LaPointe, 1991).

Seagrass communities in the Florida Keys are receiving increased nutrient loadings from a variety of land-based human activities that are accelerating coastal eutrophication.

We assessed relationships among total nitrogen and total phosphorus concentrations of the water column and the productivity, biomass, and epiphyte levels of the seagrasses,Thalassia testudinum and Halodule wrightii, along three onshore-offshore transects (Key West, Big Pine Key, and Long Key) stratified a priori into hypereutrophic, eutrophic, mesotrophic and oligotrophic communities with increasing distance from shore.

Macroalgal biomass and alkaline phosphate activity (APA) of macroalgae and attached seagrass epiphytes were also determined along the eutrophication gradients.

H. Wrightii was the dominant seagrass within inshore hypereutrophic strata whereas T. testudinium was dominant in eutrophic, mesotrophic, and oligotrophic strata.

Seagrasses at the hypereutrophic and eutrophic strata had low shoot densities, low production rates, low area biomass values, low areal production rates, but high levels of attached epiphytes and mat-forming macroalgae.

Seagrasses at the oligotrophic strata had the highest shoot densities, highest areal biomass values, highest areal production rates, and typically the lowest or second lowest epiphyte levels of all strata and typically the lowest or second lowest epiphyte levels of all strata.

Alkaline phosphatase activity was lowest for macroalgae at the offshore oligotrophic strata, and highest at the nutrient-enriched hypereutrophic strata where extensive populations of mat-forming macroalgae occurred.

Microcosm studies showed that both nitrogen and phosphate enrichment alone increased epiphyte levels and reduced rhizome growth rates in T. Testudinum whereas phosphate enrichment alone in increased epiphyte levels and reduced rhizome growth rates of H. wrightii.

Higher alkaline photphosphatase activity in macroalgae and attached blade epiphytes in hypereutrophic and eutrophic strata reflected increased phosphate-limitation in these dystrophic environments resulting from high concentrations of total nitrogen relative to total phosphate.

Sustained nutrient enrichment from land-based activities results in increased biomass of attached epiphytes and macroalgae, which attenuate light, reduce dissolved oxygen, and lead to the decline of T. testudinum and a gradient of damage from nearshore to offshore waters.

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Estuaries: The Impacts of Stormwater Discharges on Eutrophication in the Florida Keys by B.E. Lapointe, W. Matzie

01/01/2010 admin Leave a comment

THE IMPACTS OF STORMWATER DISCHARGES ON EUTROPHICATION IN THE FLORIDA KEYS

Brian E. Lapointe, Ph.D.
William R. Matzie, International Marine Research, Inc. Route 3, box 297A Big Pine Key, FL 33043 Phone (305) 872-2247

Estuaries, v19 n2B p422-435, June. 1996

EXECUTIVE SUMMARY
Storm water discharge is a major pathway by with land-based pollutants enter coastal waters. This project involved monitoring the effects of storm water inputs using four continuos-recording water quality monitoring instruments (Hydrolab Datasounde) along a nearfield transect extending from Big Pine Key to Looe Key National Marine Sanctuary (LKNMS); these instruments recorded dissolved oxygen salinity temperature, and tidal stage at 30 min. intervals. Water samples were also collected for nutrient determinations along this and another far-field transect (extending from Shark River on the southwest Florida shelf to LKNMS) to determine relationships between nutrient concentrations and salinity from both near-field and far-field watersheds.

Concentrations of nutrients, chlorophyll a, and turbidity increased along the near-field transect with increased freshwater inputs between the dry season (April-May, 1992) and the wet season (June-August, 1992). Freshwater discharges in early June resulted in anoxic conditions at the most inshore monitoring station (Port Pine Heights canal), a station directly impacted by septic tank leachate. High concentrations of total phosphorus correlated with freshwater inputs along both transects, indicating land-based runoff from the Keys and southwest Florida as nutrient sources. High concentrations (up to 21 uM) of ammonium followed rainfall events at the inshore stations; ammonium concentrations averaged > 1.0 uM along the entire near-field transect over the study period. Chlorophyll a and turbidity increased from the dry season into the wet season in response to the land-based storm water nutrient inputs.

A comparison of nutrient and chlorophyll-a concentrations in coastal waters of the Keys with threshold values for eutrophication on coral reefs suggests that waters of the Keys have entered a stage of critical eutrophication. Ammonium concentrations have increased several-fold at LKNMS over the past seven tears and total phosphorus, chlorophyll a and turbidity are currently 2-3 fold higher than on pristine coral reefs in the western Caribbean. The high levels of nutrients, chlorophyll a, and turbidity in coastal waters of the Keys demands that special precautions be exercised in the treatment and discharge of wastewaters and land-based runoff.

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Harmful Algae: Macroalgal blooms on southeast Florida coral reefs..indicate nutrient enrichment by B.E. Lapointe, et. al.

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Lapointe etal 05 1 Macroalgal Blms on SE FL Crl Rfs Nut Stoich of C isthmocladum wider Carib

This leading study documents that the invasive algal blooms of codium along the Palm Beach and Broward County coasts during the summer of 1990 and thereafter were caused by increasing land-based sources of pollution, particularly sewage critical to providing the needed nutrients for such an extensive harmful event. Published by Elsevier B.V. in Harmful Algae 4 (2005) 1092-1105.

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Rosenstiel School of Marine Science: Key to Florida Bay Locked in Ancient Coral

01/01/2010 admin Leave a comment

http://www.rsmas.miami.edu/info/pressreleases/ancient-coral.html

Key To Florida Bay Locked In Ancient Coral

AUGUST 6, 1996VIRGINIA KEY, Fla.–During the past several years, the water quality of Florida Bay, part of the Everglades ecosystem, has deteriorated at a steady rate. Since 1987 large areas of sea grass have died, algae blooms have created “dead zones” in parts of the bay, and populations of birds, juvenile fish and shrimp have declined dramatically. Many people blame this ecological imbalance on high salinity in the bay caused by the diversion of freshwater from the Everglades (and ultimately Florida Bay) to the metropolitan areas on the coasts north of the bay.

But a new University of Miami Rosenstiel School of Marine and Atmospheric Science-led study of coral skeletons from Florida Bay suggests that the present poor conditions of bay water were set in motion nearly 100 years ago by the construction of the Florida East Coast Railway, which connected Miami to Key West. By analyzing the oxygen and carbon isotopes in the skeleton of a 160-year-old coral head from the bay, scientists discovered that there has been no clear increase during the last 80 years in the salinity of the southern portion of Florida Bay from which the skeletons were retrieved.

This finding, which is presented in the journal Palaeogeography, Palaeoclimatology, Palaeoecology, runs contrary to a widely-held belief that the decline of Florida Bay is the result of high salinities caused by a man-made diversion of freshwater from the Everglades.

“There is no doubt that the flows of freshwater into Florida Bay have declined, but the geological record from this coral head shows no clear evidence that during the last 80 years this decline has substantially affected the salinity of the portion of the bay where this coral is located,” says principal investigator Dr. Peter Swart, chairman of the Division of Marine Geology and Geophysics at the Rosenstiel School.

“We suggest that the railway construction combined with a reduction in the number of hurricanes over several decades has allowed a buildup of organic material within Florida Bay.”

During construction of the railway, natural gaps between the keys in several locations were filled in to form causeways linking the islands. Swart and his colleagues believe that these unnatural fills reduced the natural exchange of water between Florida Bay and the open sea of the Florida Straits.

“These blockages allowed Florida Bay to retain more of its organic-rich muds,” Swart says. “This organic material decays, reducing levels of free oxygen in the water and causes a build-up of nutrients. The nutrients promote algal blooms, further reducing the oxygen in the bay through the process of eutrophication.”

According to Swart, this build-up of nutrients in the bay has been compounded by the fact that Florida Bay has received relatively few hurricanes during the past 47 years. “Hurricanes have a cleansing effect on the bay,” he says. “They remove organic-rich sediment to the open ocean.”

By analyzing the oxygen and carbon isotopes of cores drilled from the bay corals, Swart and his colleagues determined that there has been no clear increase in the salinity in the lower part of the bay during the past 80 years. The largest change in salinity and nutrients occurred between 1905 and 1910, coincident with the construction of the Florida East Coast Railway. Salinity in that part of the bay has not changed significantly in the years since construction of the South Florida canals that have been diverting water away from the bay.

“A combination of the changes brought about by the railway and the absence of hurricanes has caused this mess,” Swart says. He believes that, in addition to current efforts to redirect freshwater toward the bay, it may also be necessary to restore the natural exchange of water between the bay and the sea by replacing keys causeways with bridges.

The finding does have its detractors, Swart acknowledges. “Some scientists suggest that the salinity changes in the lower bay may not be representative of other portions of the bay, particularly in those areas where the most significant sea grass die-offs are taking place. But other data suggest that salinity in one portion of the bay is strongly correlated to salinity in other parts of the bay.”

Swart and his students Genny Healy and Lisa Greer, together with Richard Dodge of Nova Southeastern University are now undertaking a study for the South Florida Water Management District to correlate salinity records of corals from throughout the bay as a way to assess their current findings as a predictor of the condition of the bay as a whole.

“In many ways corals can be considered to be the trees of the sea,” Swart says. “Many of them form annual bands in their skeletons comprising various densities of calcium carbonate. By counting and measuring these bands, the age and growth rate of the coral can be determined.”

Corals serve as archives of environmental conditions of the past. By analyzing the chemical composition of coral skeletons, scientists can determine water salinity, water temperature and other factors that affected the growth of a coral during its history. Swart points out that coral cores are removed with minimal damage to the coral head. After drilling, cores are plugged with cement, which serves as substrate for the re-growth of corals and prevents damage from organisms that bore into corals.

“There is a saying in geology that the present is the key to the past,” Swart says. “In this case, the past is very much the key to the present.”

The University of Miami Rosenstiel School of Marine and Atmospheric Science ranks among the world’s premier oceanographic research and teaching institutions. The Rosenstiel School is a global leader in the study of sea and air circulation, fisheries, coral reefs, climate change, and ocean conservation. The School celebrated its 60th anniversary in 2003.